Study on toxin characteristics of <I>Clostridium difficile</I> isolated from hospital

ObjectiveTo analyze the toxin gene and drug resistance gene of IClostridium difficile/I isolated from the stool samples sent by hospitals. MethodsA total of 12 strains of IClostridium difficile/I were isolated from 112 stool samples by selective anaerobic culture, glutamic acid dehydrogenase (GDH) and API 20A (bioMerieux) identification, then ItcdA、tcdB/I genes, binary-toxin genes and clindamycin resistance gene（erm B）were detected by PCR. ResultsToxic IClostridium difficile/I was found in 8 out of 12 strains, 5 strains were ItcdAsup+/sup tcdBsup+/sup/I ( 62.5%)；3 strains were ItcdAsup-/suptcdBsup+/sup/I (37.5%); all the strains were negative for binary-toxin genes; and 4 out of 8 strains were positive for clindamycin resistance gene (erm B) (50%). ConclusionProportion of ItcdAsup-/suptcdBsup+/sup Clostridium difficile/I increased, so single toxin A detection might lead to the missed diagnosis of IClostridium difficile/I-associated diarrhea (CDAD) patients; and more attention should be paid to the drug resistance of IClostridium difficile. /I