Study on multiple locus VNTRs analysis of <I>Salmonella</I> Typhi by nucleic acid separation technology based on microfluidics

ObjectiveTo probe into and optimize the technology of multiple locus VNTRs analysis of ISalmonella/I Typhi by microfluidics-based nucleic acid separation technology. MethodsTo improve reproducibility of experiment by artificial internal markers which resemble DNA fragment analyzed. To analyze amplification products of 10 ISalmonella/I Typhi VNTR locus by Agilent 2100 analyzer and sequence. And then to compare two methods and optimize microfluidics-based nucleic acid separation technology. ResultsThe new internal markers could increase reproducibility of experiment. After electrophoresis by Agilent 2100 bioanalyzer and revise by novel internal marker, the location of ISalmonella/I Typhi strains is same on the electropherogram, the VNTR copy numbers of corresponding strains are same. The result was proved by sequence analysis. ConclusionThe optimized DNA separation technology based on microfluidics can use for Multiple locus VNTRs analysis of ISalmonella/I Typhi.