Etiological detection of a food borne cholera outbreak

ObjectiveTo report the etiological characteristics of a food borne cholera outbreak caused by IVibrio cholerae/I. MethodsLaboratory isolation and culture of bacteria and etiological identification were performed on the basis of epidemiological survey and clinical symptom. ResultsA total of 52 stool samples from feast participants and 49 outer-environmental samples were collected. Ten stool samples and 1 well water sample were positive for serotype Ogawa of IVibrio cholerae/I serogroup O1 (phage biotype: 15f, virulence gene: Ictx/I). IVibrio cholerae/I was not detected from the samples of 48 close contacts samples. The biochemical identification results of 11 strains of IVibrio cholerae/I were same. They were positive for oxidase test, ornithine decarboxylase, lysine decarboxylase, glucose, sucrose tests and negative for arginine dihydrolase, pectin sugar tests. They were moderately sensitive to amoxicillin and rifampicin, and sensitive to norfloxacin, cefotaxime, sulfamethoxazole, gentamicin and nalidixic acid. ConclusionThe food borne cholera outbreak was caused by serotype Ogawa of IVibrio cholerae/I serogroup O1 (phage biotype:15f), Norfloxacin, cefotaxime were the appropriate antibiotics for the treatment and prevention.