ZHANG Gui-sheng. Analysis on problems in application of ELISA[J]. Disease Surveillance, 2009, 24(9): 724-728. DOI: 10.3784/j.issn.1003-9961.2009.09.029
Citation: ZHANG Gui-sheng. Analysis on problems in application of ELISA[J]. Disease Surveillance, 2009, 24(9): 724-728. DOI: 10.3784/j.issn.1003-9961.2009.09.029

Analysis on problems in application of ELISA

  • This paper analyzes the problems in the application of ELISA on the establishment of blank control, the standard of negative control, the differences of ELISA plate, the incubation of the ELISA plate, zero setting and color selection of ELISA reader, the determination of cut-off value, positive and negative gray zone rate and the standardization of ELISA reagent.
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