Study on the single nucleotide polymorphism in gene of Bacillus anthracis isolated in Inner Mongolia

Objective To study the characteristics of single nucleotide polymorphism(SNP)in gene of Bacillus anthracis isolated in Inner Mongolia. Methods Twenty two Bacillus anthracis isolates from different sources were selected. The 13 SNP sites were amplified by using real-time PCR, and related clustering analysis was conducted. Results The identified isolates had the same sequence in 8 sites and different nucleotide sequence in the other 5 sites of the 13 SNP sites. The 22 Bacillus anthracis isolates were divided into 4 groups by cluster analysis. One A.Br.Aust94 strain and 1 A.Br.008/009 strain were detected. Twenty strains belonged to A.Br.Ames and A.Br.001/002 subgroups. Conclusion The analysis showed that single nucleotide genetic stability and the 5 discriminating SNP sites could be used as indicators in genotyping the Bacillus anthracis isolated in Inner Mongolia. A.Br.Ames and A.Br.001/002 subgroups were the clusters predominating in Inner Mongolia.