Isolation and identification of Arbovirus in southern part of Shanxi province, 2006

Objective To study the category and distribution of arbovirus in southern part of Shanxi province. Methods Mosquitoes were collected by lamp baited trap and then used for virus isolation by cell culture. The virus isolates were identified by serological and molecular biological method. Results A total 3436 mosquitoes were collected from Yuncheng, Linfeng and Yangquan in 2006, which belonged to 4 species of 3 groups. Five virus strains were isolated from mosquitoes. Three strains were identified as CppDNV, 2 strains need further identification. The analysis of partial nucleotides sequence of CppDNV non-structural protein 1 (NS1) and 2 (NS2) indicated that the homology between 3 CppDNVs and other Chinese CppDNV strains ranged from 99.9% to 100%. Phylogenetic tree showed that all Chinese CppDNV strains are located in one distinct branch and have close phylogenetic relationship with AaeDNV. Conclusion CppDNV was isolated for the first time in Shanxi province, which has high homology with other Chinese CppDNV strains.