Establishment of IgG capturing enzyme-linked immunosorbant assay to detect Japanese encephalitis virus antibody

Objective To establish IgG capturing enzyme-linked immunosorbant assay (ELISA) to detect Japanese encephalitis virus (JEV) antibody in human serum by using monoclonal antibody. Methods Monoclonal antibody to JEV was coated in the plate, inactivated and purified JEV antigen was added to the coated plate and then detected serum was added. The results were interpreted according to color development. Immunofluorescence assay (IFA) was used as the control method in this study. Results Compared with IFA, the sensitivity, specificity and consistency of the developed ELISA was 95.6%, 85.7% and 91.3% respectively. Its sensitivity and specificity was higher than other two commercial kits. Conclusion The developed ELISA has good proficiency and can be used to screen IgG antibody to JEV in human serum.