Detection of Japanese encephalitis virus antibodies in piglets in three gorges reservoir area

Objective To learn about the time when Japanese encephalitis virus (JEV) antibody-positive rate reached 50% among piglets in three gorges reservoir area and provide theoretical evidence for the prevention and control of Japanese encephalitis in this area. Methods The piglets serum samples were taken from four surveillance sites in three gorges reservoir area.The serum IgG antibody was detected by using of enzyme-linked immunosorbent assay method (ELISA) and the serum IgM antibody was detected by using of indirect immunofluorescence assay (IFA). The statistical analysis was conducted by using SPSS 12.0 statistical software. Results In Wanzhou and Yubei districts of Chongqing municipality,piglets infected with JEV in early May, and in mid May the positive rate of IgG antibody reached 50%, but in Fuling district of Chongqing municipality and Zigui county of Hubei province,piglets infected with JEV in late May and the positive rate of IgG antibody reached 50% in mid June. The JEV antibody positive rate of piglets raised in hoggeries was higher than those raised separately,the difference was statistically significant(2=43.797,P0.05). Conclusion In three gorges reservoir area,the time when JEV IgG antibody positive rate reached 50% was from late May to mid-June.