Detection and analysis of 5 strains of Vibrio cholerae O1 with virulence gene from green turtles and aquaculture water

Objective To detect Vibrio cholerae O1 or O139 from aquatic products in Jianggan district and identify the pathogen and molecular genotype of the strains isolated. Methods The isolation and identification of Vibrio cholerae from 51 samples were conducted according to Manual for Cholera Prevention and Control. Kirby-Bauer method was used to test the susceptibility of the isolated strains of Vibrio cholerae to 14 antibiotics, PCR was performed to detect the virulence gene-ctxA and tcpA, and PFGE was used for molecular typing. Results Five strains of Vibrio cholerae O1 were detected from the 51 samples, including 4 Ogawa strains and 1 Inaba strain. The result of PCR for virulence gene detection showed they all carried ctxA and tcpA. The Ogawa strains were sensitive to Ampicillin but the Inaba strain was resistant to it, the 5 strains were all sensitive to 11 antibiotics, and mesomeric to erythromycin and rifampicin. The Results of PFGE showed that the 5 strains could be divided into two types, since there were only one fragment difference between them, Conclusion All the 5 isolated strains of Vibrio cholerae carried virulence gene-ctxA and tcpA, The surveillance and management of aquatic products, especially green turtles, should be strengthened.