Survey on etiology of tuberculosis and drug-resistance of Mycobacterium tuberculosis in Weishi county, Henan province

Objective To investigate bacteriological prevalence situation of tuberculosis(TB)and the situation of drug resistant in Weishi,Henan. Methods The isolate strains were identified by the identification culture and multiplex polymerase chain reaction(PCR). The strains were tested drug resistant with proportion method. Results Total 2661 sputum samples were detected. 179 cases were sputum smear positive (6.5%),245 cases were culture positive(9.2%), Among them,225 strains were Mycobacterium tuberculosis (MTB.Mycobacterium tuberculosis),accounting for 91.8%,six strains were Mycobacterium complex(2.4%), 13 strains were non- tuberculosis Mycobacterium(5.3%).1 strain was co-infected by Mycobacterium tuberculosis and non-tuberculosis Mycobacterium. The drug resistant test shows 54strains were drug-resistant (24.0%),M.TB were16.9%(38/225) 16.0%(36/225),9.8%(22/225) and 8.4%(19/225)to resistant SM,INH,RFP and EMB respectively. Among drug-resistant strains, 20 strains were single drug-resistant(8.9%) ,Multi-drug-resistant strains were 19 (8.4%).The drug resistant rate differences was no statistically significant between male and female(2=2.8214,P0.05).There was statistically significant between drug resistant and age group(2=8.3868,P0.05). The drug resistant rate differences was statistically significant between retreatment cases and initial treatment cases(2=13.7193,P0.05). The Multi-drug resistant rate differences was statistically significant between retreatment cases and initial treatment cases(2=11.1361,P0.05). Conclusion It showed that the tuberculosis bacteria include the Mycobacterium tuberculosis、 Mycobacterium complex and non-tuberculosis Mycobacterium. The MTB isolates of Weishi is very serious on drug resistant, We should especially give sufficiency attention for retreatment cases and the younger group.