Technique and its application for identifying vaccine target proteins of Salmonella paratyphi A

The incidence of Salmonella paratyphi A infection in human is becoming higher than other pathogens causing typhoid/paratyphoid. The outbreaks caused by endemic S. paratyphi occur every year in some regions in China and the epidemic of typhoid is more serious in Southeast Asia. However, no vaccine against S. paratyphi A is available currently. Therefore, it is important to identify the immunogenic proteins that induce the immune system to produce antibody and develop the whole bacteria attenuated vaccines as well as subunit vaccines. Along with the release of ATCC9150 whole genome sequence of S. paratyphi A, the Methods using genome and proteomics would be useful for the study of vaccine targets aimed at S. paratyphi A. This paper summarizes the Methods for identifying the vaccine targets and the list of potential S. paratyphi A antigens which could be used in differential diagnosis and as effective vaccine targets.