Carbopenems resistant mechanism of a strain of Enterobacter cloacae

Objective To understand the carbopenems resistant mechanism of a strain of Enterobacter cloacae isolated in our hospital. Methods The minimal inhibitory concentrations of the Enterobacter cloacae strain and conjugate bacterium were detected with E-test,the carbapenemase was detected with Hodge test. Conjugation test, polymerase chain reaction (PCR), plasmid extraction, agarose gel electrophoresis and cloning and sequencing of drug resistant gene were conducted to analyze the resistant mechanism. Results Conjugation test indicated that the carbopenems resistant gene of the Enterobacter cloacae strain was in a conjugative plasmid. The gene was identified as BlaKPC-2 through gene amplification, sequencing and comparison. Conclusion The strain of carbapenem resistant Enterobacter cloacae produced KPC-2 carbapenemase.