Genotyping and drug susceptibility test of Vibrio vulnificus strains

Objective To understand the molecular epidemiological characteristics and drug susceptibility of Vibrio vulnificus in marine products. Methods Polymerase chain reaction (PCR) was conducted to carry out the nucleic acid detection and genotyping of V. vulnificus strains isolated from marine products, and the drug susceptibility of these isolates was tested by ATB method. Results All V. vulnificus strains were positive for hemolysis A gene (vvhA). Five genotypes were detected, they were CB type, CAB type, EA type, CA and EAB type. One BT2 nucleic acid positive strain and one SerE nucleic acid positive strain were detected. The drug susceptibility test results showed that these isolates were resistant to aminoglycosides and cephalosporins. Conclusion The genotypes of V. vulnificus isolates from marine products varied,but CB type predominated,EAB type and CAB type were reported for the first time in China. It is necessary to strengthen the detection of V. vulnificus in marine products and monitoring of its drug susceptibility to prevent the outbreak of V. vulnificus infection.