Detection of blaoxa-23 and ISAba1 genes in Acinetobacter baumannii and their relationship with drug resistance

Objective To understand the current status of drug resistance of Acinetobacter baumannii and the resistance induced by genome blaoxa-23. Methods The susceptibility of 200 strains of A. baumannii to common antibiotics were tested by Kirby-Bauer method, and polymerase chain reaction was conducted to detect carbapenemases-related genome blaoxa-23 and the insertion sequence of ISAba1. Results The resistance rates of cefoperazone/sulbactam, imipenem and polymyxin were relatively low, but the resistant rates to other antibiotics were all above 80%. Among the 200 strains, 155 carried blaoxa-23 gene and 168 carried the insertion sequence of ISAba1. Conclusion The drug resistance of A. baumannii in our hospital was very serious, many A. baumannii strains carried blaoxa-23 gene, which would be responsible for their drug resistance, the insertion sequence of ISAba1 was often associated with the presence of blaoxa-23 gene.