Detection of OXA-type carbapenemases genes of Aciuetobacter baumauuii

Objective To understand the carriage of OXA-type carbapenemases gene in Acinetobacter baumannii. Methods Carbapenemases genes of blaoxa-23, blaoxa-24, blaoxa-51, blaoxa-58 were tested by polymerase chain reaction (PCR) and DNA sequencing of those PCR products was conducted. The insertion sequence ISAba1, ISAba3 were tested by polymerase chain reaction and the sequencing of PCR products were conducted. Besides the detection of genes of blaoxa-23 and the insertion sequence ISAba1, another primer was designed to conduct amplification between the gene blaoxa-23 and the insertion sequence ISAba1.The overall length of DNA sequences between ISAba1 and OXA-23 was obtained. Results A total of 200 Acinetobacter baumannii strains were isolated from the patients in our hospitals, and 155 were detected to carry blaoxa-23 gene by using PCR (77.5%), and in which 143 carried the insertion sequence ISAba1 (71.5%), but no blaoxa-24 gene was detect4ed in the 200 strains. All of these strains carried blaoxa-51 gene and 156 strains also carried blaoxa-23 gene (77.5%). Eight strains carried blaoxa-58 gene (4.0%) and 12 strains carried the insertion sequence ISAba3 (6.0%) and there were 8 strains which carried both ISAba3 and blaoxa-58 (4.0%). Conclusion Carbapenemase blaoxa-23 gene producing of Acinetobacter baumannii was the main cause of the resistance to carbapenemase in Acinetobacter baumannii infection treatment in our hospital.