Molecular epidemiological survey of drug resistance gene of Pseudomonas aeruginosa in a hospital in Yunnan, China

Objective To understand the characteristics of drug resistance and molecular epidemiological characteristics of drug resistant gene of Pseudomonas aeruginosa in Yunnan province, and provide useful information for clinical antibiotic use. Methods A total of 28 P. aeruginosa strains were isolated from the patients in our hospital from June to September 2013. The identification and drug susceptibility test were conducted by using bioMerieux VITEK-2 system. The drug-resistance determents were analyzed by PCR amplification and sequencing. Results According to the standardized international definitions for drug-resistance, 22 strains (78.6%) were confirmed as multidrug-resistant (MDR), 4 (14.3%) were extensive drug resistant (XDR) and 2 (7.1%) were pan-drug resistant (PDR). All the isolates were resistant to the 1st and 2nd generation cephalosporins and most 3rd and 4th generation cephalosporins, trimethoprim-sulfamethoxazole and furadantin. Moreover, most strains (85.7%) showed carbapenem-resistant. The analysis of the resistance determinant indicated that 26 strains (92.9%) carried both quinolone and aminoglycosides resistance-associated genes, and the blaFOX/qnrB/aacA4 was predominant (21.4%, 6/28). The analysis of the gene-capturing elements showed that 16(57.1%)strains carried the int 1 gene, 6 (21.4%) carried the IS CR1 gene and 10 (35.7%) carried the ISEcp1 respectively. Multi-clone groups were found by PFGE analysis. Conclusion High positive rates of multi-clone MDR P. aeruginosa carrying -Lactamase, quinolone and aminoglycosides resistance-associated genes was observed in this study and effective control measures should be taken urgently.