CHPG protects against LPS-induced inflammation by modulating mGluR5 and JNK signaling pathway

Objective To investigate the mechanism by which mGluR5 activation suppress the inflammation induced by activated microglia. Methods The effect of (RS)-2-chloro-5-hydroxyphenylglycine (CHPG) on LPS-induced inflammation, the expression of mGluR5 and its downstream MAPK signaling pathways were detected with Griess test, enzyme-linked immunosorbent assay, western blot and flow cytometry. Results CHPG reversed LPS-induced decrease of mGluR5 (P=0.0003) and p-JNK expression (P=0.005) and no such effect of CHPG was observed in mGluR5 knockdown cells (P=0.4694, P=0.4407). Conclusion The anti-inflammation effect of mGluR5 activation is mediated by raising the expression of mGluR5 and its downstream JNK signaling pathway.