Detection of neuron specific enolase, S-100 and myelin basic protein levels in serum and cerebrospinal fluid of children with EV71 encephalitis

Objective To explore the change and its clinical significance of neuron specific enolase(NSE), S-100 and myelin basic protein(MBP) levels in serum and cerebrospinal fluid (CSF) of children with EV71 encephalitis. Methods According to Expert consensus on severe EV71 infection treatment, the sick children were divided into three group, i.e. mild case group, severe cases group and critical case group, and a control group was set. The concentrations of NSE in serum and CSF samples of children of each group in different phases were detected by electrochemiluminescence method, and the concentrations of S-100 and MBP in serum and CSF samples of children of each group in different phases were detected by double antibody sandwich ELISA. Results The serum and CSF levels of NSE and S-100 of severe cases and critical cases were significantly higher than those of the mild cases and control respectively (P0.01). The serum and CSF levels of NSE and S-100 in critical cases were higher than those in severe cases (P0.05 or P0.01). The difference in serum and CSF levels of NSE and S-100 was significant between mild cases and controls. The serum and CSF levels of NSE and S-100 of severe cases/critical cases in acute phase were significantly higher than those inconvalescencephase of severe/critical cases and the controls (P0.01), but the difference in serum and CSF levels of NSE and S-100 had no significant between severe/critical cases in convalescencephase and controls. The group specific differences in serum and CSF level of MBP had no significance. Positive correlation between NSE level and S-100 level in serum and CFS samples were observed in severe cases (r=0.886, 0.875, P0.01). Conclusion Detection of serum and CFS levels of NSE and S-100 in children with EV71 encephalitis might be helpful in assessment of brain damage and prognosis of EV71 encephalitis.