Antibiotic resistance and pulsed-field gel electophoresis molecular type of diarrhoeagenic Escherichia coli isolated in Changping district of Beijing,2015
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Graphical Abstract
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Abstract
Objective To understand the pulsed-field gel electophoresis (PFGE) molecular type and antibiotic resistances of diarrhoeagenic Escherichia coli isolated in Changping district of Beijing in 2015. Methods K-B method recommended by Clinical and Laboratory Standards Institute was used to test the drug susceptibility of the diarrhoeagenic E. coli strains. PFGE was performed according to the PulseNet protocol. Results According to the drug susceptibility test results,the diarrhoeagenic E. coli strains were completely sensitive to cefepime and imipenem,and the resistant rates toamikacin,tobramycin and minocycline were 94.6%,97.3% and 97.3%,respectively. Multidrug resistance was more serious,most strains were resistant to 2-7 kinds of antibiotics. 31 PFGE patterns were found in 33 diarrhoeagenic E. coli strains with the similarity coefficient of 68%-100%,and two Enterotoxigenic E.coli (ETEC) strains and two Enteropathogenic E.coli (EPEC) strains respectively belonged to the same pattern. Conclusion Multidrug resistance of diarrhoeagenic E.coli strains was serious in Changping,and the PFGE patterns were with highly polymorphism.
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