Genetic analysis of complete genome of two coronaviruses strains isolated in Ganzhou, Jiangxi

Objective To investigate the genetic characteristics of two strains of coronaviruses carried by Asian house shrew in Ganzhou. Methods The complete genome sequences of 2 coronavirus strains isolated from stool samples of Asian house shrew were amplified by using RT-PCR and RACE. The nucleotide sequences similarity was calculated with software MegAlign. Phylogenetic analysis was performed by using software phyML. Results The nucleotide sequences of whole genome of GZ01 strain and GZ02 strain shared the high homology (88.6%-92.1%) with other strains of coronaviruses previously detected in Asian house shrew. Phylogenetic analysis confirmed that GZ01 and GZ02 strains had the closest relationship with these strains. Conclusion Coronavirus carried by Asian house shrew from Ganzhou belonged to alphacoronaviruses.