YANG Jing, WANG Hong, ZHU Wen-tao, ZHANG Gui, JIN Dong. Antibiotic susceptibility and multilocus sequence typing of Enterococcus faecium strains isolated from foods and environment in Zigong, Sichuan[J]. Disease Surveillance, 2017, 32(12): 925-930. DOI: 10.3784/j.issn.1003-9961.2017.12.008
Citation: YANG Jing, WANG Hong, ZHU Wen-tao, ZHANG Gui, JIN Dong. Antibiotic susceptibility and multilocus sequence typing of Enterococcus faecium strains isolated from foods and environment in Zigong, Sichuan[J]. Disease Surveillance, 2017, 32(12): 925-930. DOI: 10.3784/j.issn.1003-9961.2017.12.008

Antibiotic susceptibility and multilocus sequence typing of Enterococcus faecium strains isolated from foods and environment in Zigong, Sichuan

  • Objective To investigate the antibiotic resistance and virulence gene carriage of Enterococcus faecium isolates from food and environmental samples collected from food markets in Zigong, Sichuan province, and to observe sequence typing (ST) identified by multilocus sequence typing (MLST). Methods A total of 157 food and environmental samples, which included raw meat, cooked meat and refrigerator swabs, were collected in 2013 for E.faecium isolation and the drug susceptibility tests of the isolates were performed by automatic bacteriology identification instrument. The presence of two virulence genes (hyl and esp) were detected with polymerase chain reaction (PCR), and ST types were detected by MLST analysis.Results Of 157 food samples and refrigerator swabs, 44 E.faecium strains were isolated (28.03%), in which 38 were resistant to at least one antibiotic (86.36%). The resistant rate was 65.91% for erythromycin (29/44), 31.82% for tetracycline (14/44) and 27.27% for rifampicin (12/44) respectively, while several strains showed resistance to ciprofloxacin(9.09%, 4/44),high concentration streptomycin and gentamicin (11.36%, 5/44; 9.09%, 4/44) and chloramphenicol (6.82%, 3/44). One quinupristin-dalfopristin-resistant E. faecium strain was isolated from a meat sample. Strains resistant to penicillin, glycopeptides, lipopeptides, quinolones and oxazolidinone were failed to detect in this study. Two virulence genes (esp and hyl) were negatively distributed in 44 isolates, except two isolates carrying esp gene. The MLST analysis of 44 E.faecium isolates revealed 38 different ST types, 10 strains belonged to known STs (ST94, ST301, ST69, ST361 and ST957). ST94 clonal complex was predominant, including 13 isolates. One multi-drug resistant strain E. faecium belonging to clonal complex 17(CC17) and one ST957 isolate was also detected respectively in this study.Conclusion Multi-drug resistant E.faecium isolates with different STs contaminates food and environment in Zigong,suggesting that close attention should be paid to its potential threat to human health.
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