Analysis on antibiotic resistance changes of Helicobacter pylori in Beijing,2008-2014

Objective To analyze the changes of the antibiotic resistance of Helicobacter pylori in Beijing during 2008-2014. Methods Gastric mucosa specimens were collected from the H. pylori infected patients who received gastroscopy in Beijing from May 2008 to October 2010 and from March 2013 to January 2014 respectively. After the systematic identification of isolated H. pylori strains,the resistances of the strains to 8 kinds of antibiotics,i. e. metronidazole (MZ),amoxicillin (AC), clarithromycin(CH),levofloxacin(LE),moxifloxacin(MX),rifampicin(RI),tetracycline(TC)and gentamycin(GM), were tested with E-test. The results of the two groups were compared and analyzed. Results A total of 461 and 1 848 gastric mucosa specimens were collected from the two groups of the patients(A and B),and 412 strains(89.37%)and 1 483 strains (80.25%)of H. pylori were isolated in group A and B respectively. The drug resistance test results of the 412 strains of group A and the 607 strains randomly selected in group B showed that the resistant rate of H. pylori to clarithromycin,levofloxacin, moxifloxacin and rifampicin increased significantly after 2013,the resistant rate to metronidazole decreased slightly,and the resistant rates to amoxicillin,tetracycline and gentamycin were still low. The minimal inhibitory concentration(MIC)analysis indicated that the MICs of clarithromycin,levofloxacin,moxifloxacin and rifampicin to both drug sensitive and drug resistant H. pylori strains were significantly higher after 2013. Conclusion The resistant rates of H. pylori strains to clarithromycin, levofloxacin,moxifloxacin and rifampicin were in increase in Beijing after 2013,and the MICs of the antibiotics to both drug sensitive and drug resistant H. pylori strains increased obviously,resulting in the marked decrease of clinical H. pylori eradication rate. It is imperative to adjust the breakpoints to evaluate the drug resistance of H. pylori.