Immunoproteomics research of cellular protein of Nocardia farcinica

Objective To identify the immunogenic proteins in Nocardia farcinica cellular proteins and provide evidence for the screening of specific diagnostic antigens for N. farcinica. Methods The cellular proteins of N. farcinica IFM 10152 were extracted to perform two-dimensional polyacrylamide electrophoresis and western blot assay to screen immunogenic proteins which can react with N. farcinica infected rabbit sera. Results Through immunoblotting of the cellular proteins of N. farcinica,9 proteins with antigenicity were found,in which 8 proteins had matching spots on 2-DE gel. After in-gel digestion,8 immunogenic proteins were identified by MALDI-TOF MS,which were all in cytoplasm. Conclusion A immunoproteomics assay was established in this study by which novel immunogenic proteins were found in N. farcinica cellular proteins. This assay can be used in the screening of specific diagnostic antigens for N. farcinica in the research of diagnostic reagent of N. farcinica.