Genetic analysis of Yersinia pestis strains isolated from Marmota himalayana plague focus of Qinghai-Tibet Plateau

Objective Identify the characteristics of genomovar of Yersinia pestis (Y. pestis)isolated from Naqu and Biru county in Tibet Autonomous Reagion and study the distribution of isolates with same genomovars characteristics in Qinghai-Tibet Plateau,including Tibet and Gansu,Sichuan,Yunnan provinces. Methods Analysis the molecular subtypes of 98 Y. pestis strains isolated in the plague focus of Qinghai-Tibet Plateau by 4 PCR-basic molecular subtyping methods,i.e.,Plate tectonics rearrangements,Different region (DFR),Clustered regularly interspaced short palindromic repeats (CRISPR),Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA). Results The rearrangement pattern of No.57-60 plate tectonics are the same in all Y. pestis strains isolated from Naqu and Biru county,just as the strains Z176003 (isolated in Naqu county and whole genomy was sequenced). No same genomy characterisctics were found in other strians from other regions in Qinghai-Tibet Plateau. Conclusion Strains isolated from Naqu and Biru county has its own characteristics of genome rearrangement and exclustive profiles of DRF and CRISPR. There were is a few different in VNTR loci of MLVA subtyping methods.