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Kaili Song, Yanwen Xiong, Xiunian Diao, Yujiang Guo, Yanjun Kang, Xincheng Qin, Xingwang Li. Genetic characteristics of phlebovirus carried by ticks in Xinjiang Uygur Autonomous Region[J]. Disease Surveillance, 2018, 33(8): 670-673. DOI: 10.3784/j.issn.1003-9961.2018.08.014
Citation: Kaili Song, Yanwen Xiong, Xiunian Diao, Yujiang Guo, Yanjun Kang, Xincheng Qin, Xingwang Li. Genetic characteristics of phlebovirus carried by ticks in Xinjiang Uygur Autonomous Region[J]. Disease Surveillance, 2018, 33(8): 670-673. DOI: 10.3784/j.issn.1003-9961.2018.08.014
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Genetic characteristics of phlebovirus carried by ticks in Xinjiang Uygur Autonomous Region

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    • Abstract
  • Objective To understand genetic characteristics of Phlebovirus carried by ticks in Xinjiang Uygur Autonomous Region, China.
    Methods Total RNA was extracted from tick homogenate. The screening of phlebovirus positive samples and the amplification of complete genome of phlebovirus in tick samples were performed by using reverse transcriptase polymerase chain reaction, then the genome sequences were used for sequence and phylogenetic analyses.
    Results The complete L and S segment sequences of three strains (BL10, BL33 and BL79) isolated from the tick samples in Xinjiang were amplified successfully. The 3 strains shared 98.0% — 99.7% homology with Bole Tick Virus 1. Additionally, phylogenetic analysis indicated these strains had close relationship with Bole Tick Virus 1.
    Conclusion The three strains belonged to phlebovirus, which could be identified as Bole Tick Virus 1.
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