Ruijun Yang, Xiaoguang Wang, Shiteng Huang, Bingdong Zhan, Guoping Cao, Lei Lyu, Yongliang Lei. Genetic characteristics of Chikungunya virus isolated from the first imported Chikungunya fever case in Quzhou, Zhejiang[J]. Disease Surveillance, 2018, 33(10): 875-878. DOI: 10.3784/j.issn.1003-9961.2018.10.019
Citation: Ruijun Yang, Xiaoguang Wang, Shiteng Huang, Bingdong Zhan, Guoping Cao, Lei Lyu, Yongliang Lei. Genetic characteristics of Chikungunya virus isolated from the first imported Chikungunya fever case in Quzhou, Zhejiang[J]. Disease Surveillance, 2018, 33(10): 875-878. DOI: 10.3784/j.issn.1003-9961.2018.10.019

Genetic characteristics of Chikungunya virus isolated from the first imported Chikungunya fever case in Quzhou, Zhejiang

  • ObjectiveTo understand the genetic characteristics of Chikungunya virus isolated from the first imported Chikungunya fever case with travel history in Bangladesh in Quzhou, Zhejiang province.MethodsThe serum sample was collected from the case for the detections of the nucleic acids of CHIKV and Dengue virus with real-time PCR. The gene C-E3-E2-6K-E1 fragment was amplified by using RT-PCR. The PCR products were sequenced and used for tracing analysis by using Bioinformatics software.ResultsThe positive bands of the specific nucleic acids of the CHIKV strain (QZ0823) isolated from the case could be amplified by PCR. Five structural genes of the CHIKV strain were obtained after splicing the sequencing data, including a 3 747 bp nucleotide sequence which encoded 1 248 amino acids. The nucleotide acid and amino acid of the strain shared 98.9%−99.9% and 99.0%−99.9% homologies, respectively with the reference sequences. Phylogenetic analysis of the nucleotide sequences of the E1 and C-E3-E2-6K-E1 genes revealed that the strain had close genetic relationships with the strains from Bangladesh, India, and Pakistan belonging to the genetic pedigree of East Central and South Africa.ConclusionThe CHIKV strain (QZ0823) isolated in Quzhou belonged to genetic pedigree of East Central and South Africa, indicating that the Chikungunya fever case in Quzhou was imported one.
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