Infection and etiological characteristics of Vibrio cholerae in diarrhea patients in Shunyi district of Beijing, 2015–2017

ObjectiveTo understand the infection status and etiological characteristics of Vibrio cholerae in patients with food-borne diseases in Shunyi district of Beijing, and provide evidence for effective prevention, control and treatment of V. cholera infection.

MethodsV. cholerae isolation and culture were conducted by using stool samples collected from diarrhea cases in surveillance from 2015 to 2017. PCR test of virulence genes, pulsed-field gel electrophoresis (PFGE), matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and antibiotic susceptibility analysis were carried out for the isolates of V. cholerae.

ResultsA total of 1 105 diarrhea patients were surveyed in three years. The detection rate of V. cholerae was 0.54% (6/1 105). The detection results of cholera toxin genes ctxAB were negative. Serological typing indicated that all the strains were non-O1/O139. PCR indicated that 5 of the 6 V. cholerae strains were type III secretion system (T3SS) gene positive. The clustering results of PFGE were consistent with the clustering results of MALDI-TOF MS.

ConclusionIt is necessary to attach importance to the infection and etiological surveillance of V. cholerae in food-borne disease surveillance system