ObjectiveTo understand the infection status of Campylobacter and its etiologic characteristics in diarrheal patients in autumn in Nanshan district of Shenzhen.
MethodsThe stool samples were collected from local diarrheal patients during September-November in 2018. Campylobacter was isolated with new filtration method and the species identification was performed by real time PCR. Molecular sub-typing was carried out by using multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). The minimum inhibitory concentrations (MICs) of 11 antibiotics to the isolates were obtained with agar dilution method. The genetic cluster analysis was performed by using minimum spanning tree analysis based on the sequence typing (ST) of the isolates.
ResultsA total of 150 stool samples were collected from 150 diarrheal patients, including 74 men and 76 women. The age of the patients ranged from 1 year to 86 years. Eighteen Campylobacter strains were isolated from 150 stool samples. Among the 18 isolates, 14 (77.78%) were C. jejuni positive and 4 (22.22%) were C. coli positive. Fourteen C. jejuni isolates could be classified into 11 PFGE patterns and 8 STs, and ST-9763, a novel ST identified in this study, was the predominant ST (35.71%, 5/14). All of the tested isolates were resistant to tetracycline and completely sensitive to erythromycin, azithromycin, telithromycin, gentamicin, streptomycin and clindamycin. The minimum spanning tree analysis for 14 C. jejuni strains isolated in Nanshan showed no significant clustering characteristics, the strains were distributed in groups from different sources.
ConclusionThe new filtration isolation method increased the isolation rate of Campylobacter in diarrheal patients. C. jejuni was the major species causing Campylobacter infection. The isolates in this study showed high drug resistances to tetracycline and quinolones. No significant genetic cluster was found in C. jejuni strains isolated in Nanshan district.
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