ObjectiveTo investigate and analyze the antibiotic resistance characteristics of Escherichia coli isolates from broilers.
MethodsThe pre slaughter broilers were selected from 2 large poultry farms in Hebei province in March, 2017. A total of 273 of cloacal swabs of the poultry from five different parts of the henhouses were collected. Selective agar was chosen for E. coli preliminary isolation, and VITEK®-2 Compact 60 was used for isolate identification and antibiotic susceptibility test. Extended-spectrum beta-lactamase (ESBL) producing characteristics of the isolates were analyzed simultaneously.
ResultsA total of 527 non-repetitive E. coli isolates were identified, in which 475(90.13%) isolates were ESBL-producing. The E. coli isolates showed the highest carbapenem-sensitivity (99.43%), including ertapenem, meropenem and imipenem, and the highest amoxicillin-resistance (99.24%), followed by tetracycline (94.88%), ciprofloxacin (78.94%), piperacillin (78.37%), aztreonam (77.80%), and levofloxacin (70.40%). All of the E. coli isolates were resistant to at least one group of antimicrobial agents, except for one isolate. Multidrug resistance was detected in the tested E. coli isolates (98.10%). All of the multidrug resistant E. coli isolates were ESBL producing. The most prevalent multidrug resistant isolates were those resistant to 6 groups of antimicrobial agents (35.86%, 189/527). A total of 58 multidrug resistance spectrums were obtained. The proportion of the strains with multidrug resistance to penicillins, cephalosporins, aztreonam, aminoglycosides, flouroquinolones, tetracycline, sulfamethoxazole and trimethoprim was highest (15.40%) in antibiotic resistant E. coli isolates.
ConclusionHigh prevalence of drug resistance and severe multidrug resistance were found in the tested E. coli isolates from broilers in Hebei. The multidrug resistance spectrums were complicated. It is necessary to strengthen the surveillance for E. coli of animal origin and continue the rational use of antibiotics.
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