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Fan Li, Xiaofang Guo, Qikai Yin, Xinxin Shen, Shihong Fu, Songtao Xu, Wenwen Lei, Hongning Zhou, Xuejun Ma, Huanyu Wang. Establishment of a reverse transcription recombinase-mediated amplification assay for rapid detection of Tahyna virus[J]. Disease Surveillance, 2020, 35(3): 192-196. DOI: 10.3784/j.issn.1003-9961.2020.03.004
Citation: Fan Li, Xiaofang Guo, Qikai Yin, Xinxin Shen, Shihong Fu, Songtao Xu, Wenwen Lei, Hongning Zhou, Xuejun Ma, Huanyu Wang. Establishment of a reverse transcription recombinase-mediated amplification assay for rapid detection of Tahyna virus[J]. Disease Surveillance, 2020, 35(3): 192-196. DOI: 10.3784/j.issn.1003-9961.2020.03.004
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Establishment of a reverse transcription recombinase-mediated amplification assay for rapid detection of Tahyna virus

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    • Abstract
  • ObjectiveTo establish a reverse transcription recombinase-mediated nucleic acid amplification (RT-RAA) assay for the rapid detection of Tahyna virus nucleic acid.
    MethodsPrimers and probes were designed in the conserved region of S segment of Tahyna virus. The sensitivity of the new RT-RAA assay was evaluated by constructing plasmids containing target gene fragments and cultured virus. The specificity of the assay were tested by seven viruses of flavivirus, alphavirus, Orthobunyavirus and Seadornavirus. Additionally, 30 batches of mosquito samples were used to evaluate the performance of the RT-RAA assay.
    ResultsThe limit detection of the assay was 100 copies per reaction for the constructed plasmid standard and 1 plaque-forming units (PFU) per reaction of Tahyna virus titers. There was no cross-reaction with other arboviruses.
    ConclusionA highly sensitive, specific, and easy-to-use assay was established for the rapid detection of Tahyna virus.
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