Progress in application of nucleic acid detection methods for Clostridium difficile

Clostridium difficile is an Anaerobic Gram-positive Bacillus that is found in the intestines of humans and animals. In recent years, because of abuse of antibiotics, the number of C. difficile infection has increased dramatically. As the increases of its morbidity and mortality, accurate diagnosis is important for the selection of the best treatment and preventive measures, so nucleic acid detection methods which are highly sensitive and specific, time-saving and labor-saving have been attracted more and more attention. This paper introduces and compares the applications of Food and Drug Administration (FDA)-approved and other classic laboratory-developed nucleic acid detection methods in the detection of C. difficile. provide a reference for the detection of C. difficile.