Application of micro confocal Raman technique in classification and identification of bacteria

Raman spectroscopy can be used to identify and describe the characteristics of microorganisms.Compared with the traditional microorganism isolation and culture methods and molecular biology identification technique, Raman technique has the advantages of non-culture, rapidness and high efficiency. With the combination of Raman technique and molecular biology in the field of optics, microbial identification has become more efficient and rapid. Raman microspectroscopy, combined with stable isotope probe, fluorescence in situ hybridization and optical tweezers, provides an independent method for studying the function and physiology of non-culpable microorganisms in ecosystems. The microconfocal Raman technique has a great application prospect in the identification and classification of bacteria. In this paper, the basic knowledge of Raman spectroscopy and the research progress of microconfocal Raman technique in classification and identification of bacteria are described, and the advantages and disadvantages of this technique are summarized and evaluated, in order to provide reference for the applied microbiology research of Raman technique.