Detection of pathogens in clinically diagnosed cases of bacillary dysentery in Shunyi, Beijing

  Objective  To analyze the composition and characteristics of pathogens of clinically diagnosed cases of bacillary dysentery in Shunyi, Beijing, and provide evidence for the effective prevention and control of bacillary dysentery.

  Methods  A total of 44 clinical samples were collected from clinically diagnosed bacillary dysentery cases for the detections of Shigella, Campylobacter, Salmonella, Vibrio parahaemolyticus, Vibrio cholerae, Diarrhea Escherichia coli and Yersinia enterocolitica. Serotyping, molecular subtyping and virulence gene detection were performed for the pathogenic isolates.

  Result  No Shigella and Yersinia enterocolitica were detected, and the positive rate was 30.00% (9/30) for Campylobacter, 18.18% (8/44) for Salmonella, 15.91% (7/44) for V. parahaemolyticus, 4.55% (2/44) for Diarrhea E. coli and 3.13% (1/32) for V. cholerae. Nine Campylobacter jejuni strains belonged to 9 STs; 8 Salmonella strains belonged to 4 serotypes and 7 PFGE patterns; 8 V. parahaemolyticus strains belonged to 4 serotypes and the virulence genes were tdh+/trh- and 1 V. cholerae strain belonged to non O1/O139 serotype and the virulence genes were ctx-/t3ss+.

  Conclusion  The misdiagnosis rate of bacillary dysentery was high in Shunyi, to which close attention should be paid. The detection rate of pathogenic bacteria was high in clinically diagnosed cases of bacillary dysentery and the predominant pathogens were Campylobacter and Salmonella. The rapid differential diagnosis method between Shigella and other diarrhea pathogenic bacteria should be established and applied as soon as possible.