Objective To understand the epidemiological characteristics and pulsed-field gel electrophoresis (PFGE) typing of diarrheagenic Escherichia coli (DEC) isolated from adults in Haidian district of Beijing from 2013 to 2020.
Methods Stool samples or anal swabs were collected from 1961 adults with diarrhea in 3 sentinel hospitals for DEC detection. Meanwhile, the 66 strains isolated were used for analyzed for genetic typing by PFGE.
Results A total of 187 DEC strains we're detected from 1 961 cases (9.54%). Enterotoxigenic E. coli (ETEC) and enteroaggregative E. coli (EAEC) had higher detection rates of 4.18% (82/1 961) and 3.93% (77/1 961), respectively. The proportion of astA/pic in EAEC and estIa/estIb in ETEC were highest, accounting for 79.22% (61/77) and 84.15% (69/82), respectively. The gender, age and occupation specific differences in DEC detection rate showed no significance. The proportions of patients with fever, vomiting and abdominal pain were 28.65% (53/185), 34.59% (64/185) and 78.92% (146/185) respectively. The predominant types of DEC varied in different years, the detection rate of DEC was high during June–September. No same PFGE patterns or pattern clustering were found for EAEC and Enteropathogenic E. coli (EPEC). A total of 19 PFGE patterns were detected in 23 ETEC strains indicated by cluster analysis. The similarity between these patterns was 4.84%–100.0%.
Conclusion The detection rate of DEC increased in recent years, and ETEC and EAEC were predominant. It is necessary to collect and analyze the information about the epidemiological characteristics of diarrhea patients and establish the local database of DEC PFGE pattern to provide reliable data supporting for the prevention and control of DEC infection in the future.
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