Application of FilmArray GⅠ Panel in the detection of diarrhea pathogens

  Objective   To discuss the application value of the FilmArray GⅠ Panel in the detection of diarrhea pathogen.

  Methods  152 fecal samplesfrom intestinal outpatients of Wuxi People's Hospital (including Children's Hospital) from January 2016 to September 2016 were collected. FilmArray GⅠ Panel and real-time PCR were used to detect samples, respectively, and the results of the two methods were compared.

  Results  Total of 19 pathogens were detected by FilmArray GⅠ Panel, with a positive rate of 75.00% (114/152). Total of 17 pathogens were detected by real-time PCR detected, with a positive rate of 68.42% (104/152). The coincidence rate between the two methods was 67.76% (103/152).For adenovirus, Enteroinvasive E.coli(EIEC), Campylobacter and G. lambli, the coincidence rate was 100.00%.For Sapovirus, Enteroaggregative E.coli(EAEC), C. difficile, Salmonella and V. cholerae, the coincidence rates were 98.03%, 94.08%, 96.71%, 94.08% and 98.68%, and the consistency was moderate (Kappa values were 0.563, 0.546, 0.651, 0.610, 0.744). For Norovirus GⅠ/GⅡ, Rotavirus A, Enterotoxigenic E.coli(ETEC), Enteropathogenic E.coli(EPEC), P. shigelloides and V. parahaemolyticus, the coincidence rates were 98.03%, 99.34%, 97.37%, 92.76%, 99.34%, 98.68%, and the consistency was good (Kappa values were 0.892, 0.971, 0.890, 0.764, 0.944, 0.902). Using Chi-square tests, the detection rates of EAEC, ETEC, EPEC, C. difficile and Salmonella by FilmArray GⅠ Panel were higher than those by real-time PCR, and the difference was statistically significant (P<0.05).

  Conclusion  Compared with real-time PCR, FilmArray detection has the characteristics of rapid, accurate and high throughput, which can be used for rapid detection of public health emergencies and rapid diagnosis of diarrheal diseases.