Sensitivity of Brucella melitensis isolates to antibiotics in vitro

  Objective   To study the common antibiotics susceptibility of Brucella isolates.

  Methods   This study used the broth dilution method to detect the minimum inhibitory concentration of clinically common drugs for brucellosis in clinical isolates.

  Results   This study had been found that under the current inflection point of resistance of Brucella, the Brucella isolates were all sensitive to common antibiotics. Among these, the minimum inhibitory concentration value range of doxycycline (0.06−0.12 μg/mL), tetracycline (0.06−0.25 μg/mL) and gentamicin (0.015−0.03 μg/mL) was relatively low; Analyzing the isolated ampicillin MIC values, this study found that Sichuan Province and Shaanxi Province (P=0.0001), Hebei Province (P=0.0001) and Hunan Province (P=0.04) were significantly difference, and Hebei Province and Guangdong Province (P=0.02) were significant difference. In addition, this study also found isolates with higher minimum inhibitory concentration values of erythromycin (32 μg/mL) and compound trimethoprim (0.25/4.8 μg/mL).

  Conclusion   The Brucella isolates in China involved in this study were sensitive to common clinical treatment drugs. However, some isolates were less sensitive to compound trimethoprim. Therefore, follow-up surveillance should be strengthened to prevent bacterial resistance. This study provided basic data for the monitoring of the resistance level of Brucella in China, and at the same time provided ideas for the screening, prevention and control of resistant strains.