Study on the changes of chemokine CXCL1 in prion-infected cell model

  Objective  To explore the change characteristics of chemokine CXCL1 in prion-infected cell model SMB-S15, and compare the effect of change of prion contents on CXCL1 level in prion-infected cells.

  Methods  The transcription and translation levels of CXCL1 in SMB-PS, SMB-RES and SMB-S15 cells were analyzed by fluorescence quantitative PCR, western blot and enzyme-linked immunosorbent assay (ELISA). The protein levels of CXCL1 in SMB-S15 cells without prion and with little prion treated with resveratrol and 3, 4-dihydroxyphenylene methylene acetone respectively were analyzed by ELISA.

  Results  In prion-infected SMB-S15 cells, the transcription level of CXCL1 was significantly higher than that in SMB-PS and SMB-RES cells, showing statistical difference. The expression level of CXCL1 in SMB-S15 cells was higher than that in SMB-PS and SMB-RES cells in both cell lysate and corresponding medium. The protein level of CXCL1 in SMB-S15 cells treated with resveratrol at different times and treated with 3, 4-dihydroxyphenylene methylene acetone at different concentrations all showed decreasing trendsPin a time and dose dependent manner.

  Conclusion  The level of CXCL1 significantly increased in prion-infected cells, and was positively correlated with the level of intracellular prions.