Characterization of clinical isolates of Vibrio parahaemolyticus in HuZhou, Zhejiang, 2019−2020

  Objective  To understand the characteristics of Vibrio parahaemolyticus isolated from diarrhea patients in Huzhou, Zhejiang province, from 2019 to 2020.

  Methods  A total of 109 strains of V. parahaemolyticus isolated from patients with diarrhea in Huzhou from 2019 to 2020 were collected for serological typing. Virulence genes were detected by fluorescence PCR. Drug resistance was detected by broth dilution method, and molecular typing was performed by pulsed-field gel electrophoresis (PFGE).

  Results  The predominant serotype of the 109 clinical strains was O3∶K6 (72 strains). All the strains carried gene tlh, and only two of them carried trh. There were 108 strains which were divided into 72 PFGE types by Not Ⅰ enzyme, and the similarity coefficient between different types was 19.10%–100%. The antibiotic resistance test indicated that the isolates were highly resistant to sulfamethoxazole (63.30%), followed by ampicillin (49.54%).

  Conclusion  Strengthening the continuous surveillance for V. parahaemolyticus in diarrhea patients can facilitate the risk assessment of foodborne illnesses caused by V. parahaemolyticus and provide reference for the prevention, control and clinical treatment of intestinal infection.