Drug resistance and drug resistance genes of Mycobacterium tuberculosis in three surveillance areas in Jiangxi

  Objective  To understand the drug resistance and drug resistance gene mutations of Mycobacterium tuberculosis isolates from three national tuberculosis (TB) drug resistance surveillance areas of Dayu, Jinxian counties and Guangfeng district of Jiangxi province, and provide scientific evidence for the prevention and treatment of drug-resistant tuberculosis.

  Methods  The drug susceptibility of the Mycobacterium tuberculosis in three surveillance areas in Jiangxi was detected by proportional drug sensitivity test and the gene mutations were identified by PCR-linear hybridization enzyme method.

  Results  A total of 280 TB cases from the 3 surveillance areas were included in the study, and 264 strains were isolated, including 250 M. tuberculosis strains and 14 strains of non-tuberculosis mycobacteria. For the M. tuberculosis strains, the overall dug-resistance rate was 25.60% (64/250), the mono-drug resistance rate was 7.20%(18/250), the poly-drug resistance rate was 3.20% (8/250), and the rate of MDR was 5.60% (14/250). The detection rate of drug resistance in men was higher than that in women. S315T1 was the main feature of isoniazid resistance gene mutation in this area, and gyrA gene mutation was the main mechanism of M. tuberculosis resistance to fluoroquinolones in Jiangxi.

  Conclusion  The use of PCR-linear hybridization enzyme method to screen first-line drug and second-line drug resistance gene mutations was of great significance for determining treatment options and the prevention and control of TB. It is necessary to strengthen the management of drug resistant TB cases and control the infection sources to reduce the incidence and spread of drug resistant TB in Jiangxi.