Drug resistance and gene mutation of clinical isolates of Mycobacterium tuberculosis in Lishui, Zhejiang

  Objective  To study the drug resistance characteristics and drug resistance gene mutations of 242 Mycobacterium tuberculosis clinical isolates from a designated tuberculosis hospital in Lishui and provide evidence for the prevention and control of drug-resistant tuberculosis in Lishui.

  Methods  The drug resistance and resistance gene mutation characteristics of the M. tuberculosis clinical isolates in Lishui were detected by fluorescence PCR melting curve method.

  Results  The overall drug resistance rate of 242 M. tuberculosis strains was 16.53% (40/242), and the multidrug resistance rate was 4.96% (12/242), and the resistance to isoniazid was highest, followed by streptomycin, rifampicin, fluoroquinolones and ethambutol. The mutation site of streptomycin resistance gene mainly occurred at codon rpsL43, the mutation rate was 65.00% (13/20); the mutation site of isoniazid resistance gene mainly occurred at katG315 codon , the mutation rate was 76.19% (14/21); the rifampicin resistance gene mutation sites mainly occurred at codons 529–533 of the rpoB gene, with a mutation rate of 61.11% (11/18); The mutation site of ethambutol resistance gene mainly occurred at codon 306 of embB, and the mutation rate was 77.78% (7/9).

  Conclusion  The overall drug resistance rate in tuberculosis patients in Lishui is lower than the national average levels, but the drug resistance patterns and drug resistance gene mutations are still complex and diverse, and the clinical prevention and control of drug-resistant tuberculosis needs to be further strengthened.