Application of ND-O-BSA-ELISA in detection of antibody level in family members and neighbors of leprosy patients in Guiyang, Guizhou

  Objective  To understand the serum antibody level of family members and neighbors of leprosy patients and provide evidence for the follow-up in family members and neighbor of leprosy patients.

  Methods  The general information of the study subjects and the detection data of IgM, MMP-IgG and LID-IgG by ND-O-BSA-ELISA were entered into EXCEL electronic database, and then the detection data were analyzed by χ² test and t test with software SPSS 18.0.

  Results  Three family members of leprosy patients were IgM positive, no positive cases were detected in the neighbors, but there was no significant difference in the change of IgM between the two groups (P>0.05); In the family members of leprosy patients, 50 were MMP-IgG positive, accounting for 23.47%, and 44 were LID-IgG positive, accounting for 20.66%. There were 12 MMP-IgG positive cases, accounting for 4.90%, and 11 LID-IgG positive cases, accounting for 4.90%, in the neighbors of leprosy patients. The differences in changes of MMP-IgG and LID-IgG in the two groups were significant (P<0.001).

  Conclusion  ND-O-BSA-ELISA can effectively detect the level of leprosy specific antibody in the family members and neighbors of leprosy patients. In the physical examination and follow-up, attention should be paid not only to the patients, but also to their family members and neighbors.