Contamination of Vibrio parahaemolyticus in fishery products in Xiacheng district in Hangzhou

Objective To understand the contamination of Vibrio parahaemolyticus (VP)in fishery products in farm produce markets in Xiacheng district. Methods A total of 100 samples of fishery products were collected from these markets to conduct VP isolation and identification according to the national standard. Serotyping, drug resistance test and PCR detection of species-specific gene (R72H) and virulence genes (tdh, trh) were conducted for the isolated VP strains. Results Twenty four strains of VP were isolated. All were positive for species-specific gene R72H, 22 strains were negative for virulence genes tdh and trh and 2 strains were positive for tdh but negative for trh. Conclusion VP had been the major pathogens to cause food poisoning in the area, although most VP isolates carried no tdh, trh virulence genes in this detection. It is still necessary to strengthen the surveillance of VP in fishery products.