Hydrolysis enzyme of carbapenems carrying in multidrug-resistant Acinetobacter baumannii

Objective To understand the carriage of Metal -lactamase (IMP, VIM), KPC enzyme, OXA-23 type hydrolysis enzyme in multidrug-resistant Acinetobacter baumannii in our hospital and explore the effect of these hydrolysis enzyme in carbapenems antibiotic-resistant bacteria. Methods By using Whonet 5.4, the distribution and drug susceptibility data of multidrug-resistant Acinetobacter baumannii were analyzed. By designing the specific primers, IMP, VIM, KPC and OXA-23 genes were amplified by PCR and their products were analyzed by agarose electrophoresis. Results OXA-23 gene was detected in 58 of 70 strains of multidrug-resistant Acinetobacter baumannii, the positive rate was 82.86%. No IMP,VIM and KPC genes were detected. Conclusion OXA-23 type hydrolysis enzyme was an important factor to cause the common clinical carbapenems antibiotic-resistance of Acinetobacter baumannii in our hospital.