Rapid detection of non O1 and non O139 Vibrio cholerae and their virulence genes in Zhejiang province

Objective To establish a rapid detection assay to detect non O1 and non-O139 Vibrio cholerae in sea foods and their virulence genes in Zhejiang province. Methods Non O1 and non O139 Vibrio cholerae strains were detected by using triple sugar tube, oxidase test, ropiness test and saltless peptone water test, then OmpW,ctxA、hlyA and toxR genes of Vibrio cholerae were detected by PCR assay. Results Totally 504 sample were positive by using triple sugar tube, in addition, 434 samples were positive in other 3 tests. Totally 388 strains were OmpW gene positive, but ctxA gene negative and 225 strains were both hlyA and toxR gene positive. Conclusion The established rapid assay could increase the detection rate of non O1 and non O139 Vibrio cholerae, shorten the detection time and reduce test cost. Non O1 and non-O139 Vibrio cholerae generally exist in sea foods in Zhejiang province. It is important to fully understand their pathogenicities and strengthen the disease surveillance.