Abstract: Objective To investigate the distribution and antibiotic resistance of Aeromonas isolated from the patients with acute diarrhea in Pudong New Area, Shanghai. Methods In 2014, Eleven hospitals were selected as sentinels in Pudong. The stool samples of diarrheal patients in 11 sentinel hospitals in Pudong were collected for the detection of diarrheal pathogens, including Aeromonas. The isolates were further detected for the susceptibility to 12 antibiotics with K-B method. Results A total of 73 Aeromonas infections were detected in 1846 samples (4.0%), including 42 single infections(57.6%) 8 A. hydrophila infections, 26 A. veronii bv. sobria infections, 8 A. caviae infections, and 31 co-infections with other pathogens(42.4%). A. veronii bv. sobria was the predominant pathogen (P0.05). The infection peak was during June-August (9.0%), the positive rates between males and females had no statistical significance, and the cases in age groups 0~4 years and 40-91 years accounted for 65.8%(48/73). Most Aeromonas strains were resistant to at least one antibiotic. The resistance rate to amoxicillin-clavulanic acid was highest(38.4%), followed by that to tetracycline(34.2%).Three MDR strains of A.veronii bv. sobria were detected, including 1 strain resistant to cefotaxime, ciprofloxacin, levofloxacin and other 3 antibiotics. Conclusion Aeromonas caused diarrhea had obviously seasonal/foodborne infection characteristics. High proportion of co-infections occurred. People aged 5 years and 40 years were susceptible. A.veronii bv.sobria was the predominant pathogen with increased MDR level.