Abstract: Objective To investigate the infection status of group A Rotavirus in a hospital in Yuhang, Zhejiang province, and provide scientific evidence for the prevention and control of Rotavirus diarrhea. Methods Rotavirus detection was conducted by using the stool samples collected from 42 736 diarrhea patients in a hospital in Yuhang from 2008 to 2014. Group A Rotavirus detection kit (latex method) was used. Results The overall detection rate of group A Rotavirus was 11.89% (5080/42 736), and the detection rate increased year by year from 2009 to 2013, the increasing rate between 2011 and 2012 was highest (5.01%). The detection rate of Rotavirus was 11.46% in males(2849/24 865) and 12.48% in females (2231/17 871), the difference was significant (2=10.45, P0.01). The positive detections of Rotavirus were mainly observed during October-January, accounting for 72.07% of the total (3661/5080). The annual detection peak was either in November or in December. Rotavirus was detected in all age groups. The positive detection rate in age group5 years (12.32%) was approximately 4 times higher than that in age group5 years(3.58%), and the positive rate was highest in age group 1-2 years (23.48%). The detection rate was significantly higher in out patients (12.30%) than in hospitalized patients (9.93%). Conclusion The infection rate of group A Rotavirus in a hospital in Yuhang was lower than that in Hangzhou and other parts of Zhejiang. Infants aged5 years are key population for prevention and treatment of Rotavirus diarrhea in hospitals, and the surveillance for adult Rotavirus infection should be strengthened. Diarrhea patients seeking medical care in out patient department is the key population for the management of Rotavirus diarrhea in hospitals, and the isolation and treatment of these patients should be strengthened.