一株精氨酸双水解酶阴性河弧菌的分子遗传学分析

吴睿 梁璞 卢昕 阚飙 梁未丽

吴睿, 梁璞, 卢昕, 阚飙, 梁未丽. 一株精氨酸双水解酶阴性河弧菌的分子遗传学分析[J]. 疾病监测, 2014, 29(3): 181-185. doi: 10.3784/j.issn.1003-9961.2014.03.005
引用本文: 吴睿, 梁璞, 卢昕, 阚飙, 梁未丽. 一株精氨酸双水解酶阴性河弧菌的分子遗传学分析[J]. 疾病监测, 2014, 29(3): 181-185. doi: 10.3784/j.issn.1003-9961.2014.03.005
WU Rui, LIANG Pu, LU Xin, KAN Biao, LIANG Wei-li. Molecular genetic analysis of an arginine dihydrolase-negative Vibrio fluvialis strain[J]. Disease Surveillance, 2014, 29(3): 181-185. doi: 10.3784/j.issn.1003-9961.2014.03.005
Citation: WU Rui, LIANG Pu, LU Xin, KAN Biao, LIANG Wei-li. Molecular genetic analysis of an arginine dihydrolase-negative Vibrio fluvialis strain[J]. Disease Surveillance, 2014, 29(3): 181-185. doi: 10.3784/j.issn.1003-9961.2014.03.005

一株精氨酸双水解酶阴性河弧菌的分子遗传学分析

doi: 10.3784/j.issn.1003-9961.2014.03.005
基金项目: 

国家自然科学基金(No. 81071410);十二五国家科技重大专项课题(No. 2013ZX10004-101002)

Molecular genetic analysis of an arginine dihydrolase-negative Vibrio fluvialis strain

Funds: 

This study was supported by the fund for the National Natural Science Foundation of China (No. 81071410) and the National Science and Technology Major Project for Communicable Disease Control and Prevention (No. 2013ZX10004-101002)

  • 摘要: 目的 赖氨酸、鸟氨酸脱羧酶阴性、精氨酸双水解酶阳性和阿拉伯糖发酵实验是区分河弧菌和其他非凝集弧菌的特征性化学指标。本研究对发现的1株精氨酸双水解酶阴性的河弧菌Ma-2531进行了分子遗传学分析。方法 利用多对特异性引物对精氨酸双水解酶代谢通路的arc操纵子区基因簇进行普通聚合酶链反应(PCR)扩增,并对产物进行测序分析,同时比较Ma-2531和精氨酸双水解酶阳性菌株EF85003的生长曲线和培养液pH值的变化。结果 针对arcBCA基因簇序列的3对引物arc-F/arc-R、arc-F/arc-rev和arc-ck-up/arc-R均为阴性扩增,而其上下游序列特异性的引物对arc1-up/arc1-dn和arc72695-up/arc75219-dn分别扩增出预期大小的目的条带。arc1-dnRev/arc72695-upRev 引物对的扩增产物序列分析显示该扩增片段包含transposase IS4序列,进一步的对比分析表明包括精氨酸双水解酶系统arcBCAD基因簇及上游的天冬氨酸氨甲酰转移酶调节亚基和催化亚基编码基因在内的约8.6 kb的大片段发生了缺失。生长曲线和pH值测定显示菌株Ma-2531和EF85003没有明显差异。结论 菌株Ma-2531中插入序列的转座导致了该菌株精氨酸双水解酶这一鉴定生化指标阴性的结果,但这种变化应只是随机和罕见的,使河弧菌生化代谢表现出多样性。
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出版历程
  • 收稿日期:  2014-01-22
  • 刊出日期:  2014-03-20

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