重组表达的猪溶素及其突变体诱导小鼠巨噬细胞的细胞毒性和IL-1释放

任志鸿 黄元铭 王淑京 杜华茂 宋利琼

任志鸿, 黄元铭, 王淑京, 杜华茂, 宋利琼. 重组表达的猪溶素及其突变体诱导小鼠巨噬细胞的细胞毒性和IL-1释放[J]. 疾病监测, 2014, 29(4): 276-280. doi: 10.3784/j.issn.1003-9961.2014.04.007
引用本文: 任志鸿, 黄元铭, 王淑京, 杜华茂, 宋利琼. 重组表达的猪溶素及其突变体诱导小鼠巨噬细胞的细胞毒性和IL-1释放[J]. 疾病监测, 2014, 29(4): 276-280. doi: 10.3784/j.issn.1003-9961.2014.04.007
REN Zhi-hong, HUANG Yuan-ming, WANG Shu-jing, DU Hua-mao, SONG Li-qiong. Suilysin and its mutant induced cytotoxicity and IL-1 release in mouse macrophages[J]. Disease Surveillance, 2014, 29(4): 276-280. doi: 10.3784/j.issn.1003-9961.2014.04.007
Citation: REN Zhi-hong, HUANG Yuan-ming, WANG Shu-jing, DU Hua-mao, SONG Li-qiong. Suilysin and its mutant induced cytotoxicity and IL-1 release in mouse macrophages[J]. Disease Surveillance, 2014, 29(4): 276-280. doi: 10.3784/j.issn.1003-9961.2014.04.007

重组表达的猪溶素及其突变体诱导小鼠巨噬细胞的细胞毒性和IL-1释放

doi: 10.3784/j.issn.1003-9961.2014.04.007
基金项目: 

传染病预防控制国家重点实验室出国人员留学启动基金(No.2011SKLID301)和国家自然科学基金(No.81371761)

Suilysin and its mutant induced cytotoxicity and IL-1 release in mouse macrophages

Funds: 

This study was supported by the Start-up fund for Study Abroad Returnees of State Key Laboratory for Communicable Disease Prevention and Control (No. 2011SKLID301) and the National Natural Science Foundation of China (No. 81371761)

  • 摘要: 目的 观察猪溶素(Suilysin,SLY)及其无溶血活性的突变体诱导小鼠腹腔巨噬细胞(peritoneal macrophage,PM)释放IL-1和细胞毒的差异,为进一步研究SLY诱导炎性体激活通路奠定基础。方法 重组表达纯化SLY及第353氨基酸位点突变体rSLY353L,体外和小鼠PM相互作用,用乳酸脱氢酶释放法检测细胞毒作用,用酶联免疫吸附试验(ELISA)检测IL-1的水平。结果 不同浓度rSLY(0.1~10 g)诱导小鼠PM细胞毒性呈现明显的剂量效应,rSLY(1 g/ml)诱导产生750 pg/ml的IL-1同时细胞毒20%;rSLY(5 g/ml)可引起最大量的IL-1释放但细胞毒高达90%。相同剂量的rSLY353L不能诱导可检测到的IL-1和细胞毒性。rSLY与PM共孵育20 h IL-1产量最高,20 h后IL-1产量下降但细胞毒性随孵育时间延长而增大。结论 1 g/ml SLY和PM作用20 h可诱导较高的IL-1产生同时细胞毒性较低,该实验条件适用于进一步进行SLY激活PM炎性体信号通路的机制研究。
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出版历程
  • 收稿日期:  2014-01-24
  • 刊出日期:  2014-04-20

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