耐多药结核分枝杆菌耐药相关基因突变特征分析

陈燕 赵丽丽 孙庆 赵秀芹 吴移谋 万康林

陈燕, 赵丽丽, 孙庆, 赵秀芹, 吴移谋, 万康林. 耐多药结核分枝杆菌耐药相关基因突变特征分析[J]. 疾病监测, 2014, 29(4): 305-309. doi: 10.3784/j.issn.1003-9961.2014.04.014
引用本文: 陈燕, 赵丽丽, 孙庆, 赵秀芹, 吴移谋, 万康林. 耐多药结核分枝杆菌耐药相关基因突变特征分析[J]. 疾病监测, 2014, 29(4): 305-309. doi: 10.3784/j.issn.1003-9961.2014.04.014
CHEN Yan, ZHAO Li-li, SUN Qing, ZHAO Xiu-qin, WU Yi-mou, WAN Kang-lin. Characteristics of drug resistance associated mutations in multi-drug resistant Mycobacterium tuberculosis[J]. Disease Surveillance, 2014, 29(4): 305-309. doi: 10.3784/j.issn.1003-9961.2014.04.014
Citation: CHEN Yan, ZHAO Li-li, SUN Qing, ZHAO Xiu-qin, WU Yi-mou, WAN Kang-lin. Characteristics of drug resistance associated mutations in multi-drug resistant Mycobacterium tuberculosis[J]. Disease Surveillance, 2014, 29(4): 305-309. doi: 10.3784/j.issn.1003-9961.2014.04.014

耐多药结核分枝杆菌耐药相关基因突变特征分析

doi: 10.3784/j.issn.1003-9961.2014.04.014
基金项目: 

国家自然科学基金(No.81201348);国家科技重大专项(No.2013ZX10003002-001)

Characteristics of drug resistance associated mutations in multi-drug resistant Mycobacterium tuberculosis

Funds: 

This study was supported by the National Natural Science Foundation of China for the Research of Application of MAS-PCR in Diagnosis of MDR and XDR Tuberculosis (No. 81201348) and the National Science and Technology Key Project for the Prevention and Control of AIDS, Viral Hepatitis and Other Priority Communicable Diseases (No. 2013ZX10003002-001)

  • 摘要: 目的 了解中国耐多药结核分枝杆菌耐药相关基因的分子特征。方法 对138株耐多药结核分枝杆菌和45株敏感菌的耐药相关基因inhA、katG和oxyR-ahpC间隔区(异烟肼)、rpob(利福平)、gyrA(氧氟沙星)和rrs(卡那霉素)进行序列测定,分析其基因突变特点。结果 138株耐多药结核分枝杆菌中,14.4%的菌株inhA基因发生突变,72.5%菌株的katG基因发生突变,15.9%菌株的oxyR-ahpC基因发生突变,同时考虑这3种基因,异烟肼耐药相关基因突变检出率可达90.6%;94.2%菌株的rpoB基因发生突变,74.5%菌株的gyrA基因发生突变,61.1%菌株的rrs基因发生突变,主要的突变位点为katG315(66.7%),inhA-15(9.4%),oxyR-ahpC-10(5.1%),rpoB516(13.8%),526(26.1%)和531(49.3%),gyrA90(21.6%)和94(51%),rrs1401(61.1%)。结论 我国耐多药结核菌异烟肼、利福平、氧氟沙星和卡那霉素耐药相关基因最常见突变为katG315、inhA-15,rpoB531、526和516,gyrA94和90,rrs1401。
  • [1] CanettiG,GrossetJ,HauduroY,etal.Mycobacteria:LaboratoryMethodsforTestingDrugSensitivityandResistance[J].BullWldHlthOrg,1963,9:565-578.
    [2] ZhaoY,XuS,WangL,etal.Nationalsurveyofdrug-resistanttuberculosisinChina[J].NEnglJMed,2012,366(23):2161-2170.
    [3] SomervilleW,ThibertL,SchwartzmanK,etal.ExtractionofMycobacteriumtuberculosisDNA:aquestionofcontainment[J].JClinMicrobiol,2005,43(6):2996-2997.
    [4] FloraS,FloraMS.RelationshipbetweenSbustanceAbuseandMultidrug-resistanttuberculosis[J].IbrahimMedCollJ,2012,6(2):50-54.
    [5] YuanX,ZhangT,KawakamiK,etal.Molecularcharacterizationofmultidrug-andextensivelydrug-resistantMycobacteriumtuberculosisstrainsinJiangxi,China[J].JClinMicrobiol,2012,50(7):2404-2413.
    [6] CampbellPJ,MorlockGP,SikesRD,etal.Moleculardetectionofmutationsassociatedwithfirst-andsecond-linedrugresistancecomparedwithconventionaldrugsusceptibilitytestingofMycobacteriumtuberculosis[J].AntimicrobAgentsCh,2011,55(5):2032-2041.
    [7] EngstromA,MorcilloN,ImperialeB,etal.Detectionoffirst-andsecond-linedrugresistanceinMycobacteriumtuberculosisclinicalisolatesbypyrosequencing[J].JClinMicrobiol,2012,50(6):2026-2033.
    [8] LuoT,ZhaoM,LiX,etal.Selectionofmutationstodetectmultidrug-resistantMycobacteriumtuberculosisstrainsinShanghai,China[J].AntimicrobAgentsCh,2010,54(3):1075-1081.
    [9] HuY,HoffnerS,WuL,etal.Prevalenceandgeneticcharacterizationofsecond-linedrug-resistantandextensivelydrug-resistantMycobacteriumtuberculosisinRuralChina[J].AntimicrobAgentsCh,2013,57(8):3857-3863.
    [10] ZhaoLL,ChenY,LiuHC,etal.MolecularCharacterizationofMultidrug-ResistantMycobacteriumtuberculosisIsolatedfromChina[J].AntimicrobAgentsCh,2014,AAC:01792-13.
    [11] ZhangZ,LuJ,WangY,etal.Prevalenceandmolecularcharacterizationoffluoroquinolone-resistantMycobacteriumtuberculosisisolatesinChina[J].AntimicrobAgentsCh,2014,58(1):364-369.
    [12] SinghM,JadaunGP,Ramdas,etal.EffectofeffluxpumpinhibitorsondrugsusceptibilityofofloxacinresistantMycobacteriumtuberculosisisolates[J].IndianJMedRes,2011,133:535-540.
    [13] WolfeLM,MahaffeySB,KruhNA,etal.ProteomicdefinitionofthecellwallofMycobacteriumtuberculosis[J].JProteomeRes,2010,9(11):5816-5826.
    [14] AliA,HasanR,JabeenK,etal.CharacterizationofmutationsconferringextensivedrugresistancetoMycobacteriumtuberculosisisolatesinPakistan[J].AntimicrobAgentsCh,2011,55(12):5654-5659.
    [15] DuQ,DaiG,LongQ,etal.MycobacteriumtuberculosisrrsA1401Gmutationcorrelateswithhigh-levelresistancetokanamycin,amikacin,andcapreomycininclinicalisolatesfrommainlandChina[J].DiagnMicrobiolInfectDis,2013,77(2):138-142.
    [16] ZaunbrecherMA,SikesRDJr,MetchockB,etal.OverexpressionofthechromosomallyencodedaminoglycosideacetyltransferaseeisconferskanamycinresistanceinMycobacteriumtuberculosis[J].ProcNatlAcadSciUSA,2009,106(47):20004-20009.
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出版历程
  • 收稿日期:  2014-02-16
  • 刊出日期:  2014-04-20

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