大肠埃希菌产超广谱-内酰胺酶基因型及毒力因子相关性研究

徐春泉 吴庆 张雪青 陈栎江 王赛芳 虞丹丹

徐春泉, 吴庆, 张雪青, 陈栎江, 王赛芳, 虞丹丹. 大肠埃希菌产超广谱-内酰胺酶基因型及毒力因子相关性研究[J]. 疾病监测, 2014, 29(4): 316-320. doi: 10.3784/j.issn.1003-9961.2014.04.016
引用本文: 徐春泉, 吴庆, 张雪青, 陈栎江, 王赛芳, 虞丹丹. 大肠埃希菌产超广谱-内酰胺酶基因型及毒力因子相关性研究[J]. 疾病监测, 2014, 29(4): 316-320. doi: 10.3784/j.issn.1003-9961.2014.04.016
XU Chun-quan, WU Qing, ZHANG Xue-qing, CHEN Li-jiang, WANG Sai-fang, YU Dan-dan. Relationship between genotype of extended spectrum -lactamases (ESBLs) and virulence factors of ESBLs producing E. coli strains[J]. Disease Surveillance, 2014, 29(4): 316-320. doi: 10.3784/j.issn.1003-9961.2014.04.016
Citation: XU Chun-quan, WU Qing, ZHANG Xue-qing, CHEN Li-jiang, WANG Sai-fang, YU Dan-dan. Relationship between genotype of extended spectrum -lactamases (ESBLs) and virulence factors of ESBLs producing E. coli strains[J]. Disease Surveillance, 2014, 29(4): 316-320. doi: 10.3784/j.issn.1003-9961.2014.04.016

大肠埃希菌产超广谱-内酰胺酶基因型及毒力因子相关性研究

doi: 10.3784/j.issn.1003-9961.2014.04.016

Relationship between genotype of extended spectrum -lactamases (ESBLs) and virulence factors of ESBLs producing E. coli strains

  • 摘要: 目的 分析温州医科大学附属第一医院临床分离产超广谱-内酰胺酶(Extended Spectrum Beta-Lactamase,ESBLs)大肠埃希菌耐药情况,研究大肠埃希菌超广谱-内酰胺酶的不同基因型与所含的毒力因子的关系。方法 普通聚合酶链反应(polymerase chain reaction,PCR)检测不同标本来源大肠埃希菌的毒力因子papC、sfa/foc、afa/dra、iutA、 aer、fimH、hly、cnf1、kpsMTⅡ、fyuA;纸片扩散确证法检测超广谱-内酰胺酶,PCR方法检测ESBLs不同基因型的分布情况。结果 产ESBLs大肠埃希菌株中,毒力因子iutA、fyuA的检出率较高,均50%;毒力因子fimH、aer的检出率为20%~40%,而毒力因子papC、sfa/foc、afa/dra和kpsMTⅡ的检出率均较低20%;经2检验产ESBLs菌株与非产ESBLs菌株的毒力因子分布率差异均无统计学意义(P0.5)。92株产ESBLs大肠埃希菌中,毒力因子papC在CTX-M-9群中的阳性率高于TEM群和CTX-M-1群,差异有统计学意义(P0.05);毒力因子aer在CTX-M-1群中的阳性率高于TEM群和CTX-M-9群,差异有统计学意义(P0.05)。结论 各种毒力因子检出率与细菌是否产ESBLs无相关性。但ESBLs阳性菌株中不同的基因型与某些毒力因子的存在与否存在一定关系,毒力因子与产CTX-M酶大肠埃希菌耐药情况可能有关。
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出版历程
  • 收稿日期:  2014-01-22
  • 刊出日期:  2014-04-20

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